Search results for " clearance rate"

showing 10 items of 27 documents

Effect of acyl-CoA oxidase activity on the accumulation of gamma-decalactone by the yeast Yarrowia lipolytica: a factorial approach.

2007

International audience; beta-Oxidation is a cyclic pathway involved in the degradation of lipids. In yeast, it occurs in peroxisomes and the first step is catalyzed by an acyl-CoA oxidase (Aoxp). The yeast Yarrowia lipolytica possesses several genes (POX) coding for Aoxps. This study is based on the factorial analysis of results obtained with the many POX derivative strains that have been constructed previously. The effect of interactions between Aoxps on the acyl-CoA oxidase (Aox) activity was important even at the second order. We then investigated the effect of Aox activity on growth and lactone production. Aox activity was correlated with acidification of the medium by cells and with ce…

0106 biological sciencesYarrowia lipolyticaMESH: Enzyme ActivationMetabolic Clearance RateMESH: Factor Analysis StatisticalYarrowiaBiologymodèleModels Biological01 natural sciencesApplied Microbiology and BiotechnologyLactones03 medical and health sciencesEnzyme activatorSpecies SpecificityMESH: Computer Simulation010608 biotechnologyCombinatorial Chemistry TechniquesAcyl-CoA oxidaseMESH: Species SpecificityComputer Simulation030304 developmental biologychemistry.chemical_classificationMESH: Metabolic Clearance Rate0303 health sciencesOxidase testmétabolisme des acides grasAcyl-CoA oxidase activityMESH: Acyl-CoA OxidaseMESH: Models BiologicalYarrowia[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyGeneral MedicinePeroxisomebiology.organism_classificationYeastEnzyme ActivationBiochemistrychemistrylactoneMolecular MedicineMESH: Combinatorial Chemistry TechniquesMESH: YarrowiaAcyl-CoA OxidaseFactor Analysis StatisticalLactoneMESH: Lactones
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Dose-dependent absorption and elimination of cefadroxil in man.

1991

The pharmacokinetic behaviour of cefadroxil was dose-dependent in healthy male volunteers following the oral administration of single doses of 5, 15, and 30 mg.kg-1. As the dose of cefadroxil increased from 5 to 15 and 30 mg.kg-1, the peak plasma concentrations, normalized to 5 mg.kg-1, decreased significantly from 15.1 to 10.7 and 7.6 mg.l-1, while the corresponding normalized areas under the plasma concentration-time curves from 0 to 2 h decreased significantly from 1258 to 946 and 801 min.mg.l-1. When the same subjects were given 5 mg.kg-1 of cefadroxil together with 45 mg.kg-1 of cephalexin, the absorption of cefadroxil was slowed to a similar or greater extent than with the high dose o…

AdultMaleAdolescentmedicine.drug_classMetabolic Clearance RateAntibioticsAbsorption (skin)PharmacologyKidneyAbsorptionPharmacokineticsOral administrationReference ValuesmedicineHumansPharmacology (medical)Drug InteractionsPharmacologyCephalexinDose-Response Relationship DrugChemistryCefadroxilKidney metabolismGeneral MedicineDrug interactionDose–response relationshipCefadroxilmedicine.drugEuropean journal of clinical pharmacology
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'Prefrontal' cognitive performance of healthy subjects positively correlates with cerebral FDOPA influx: an exploratory [18F]-fluoro-L-DOPA-PET inves…

2006

Dopamine neurotransmission influences those cognitive processes, which are generally regarded as prefrontal cortical functions. In previous positron‐emission‐tomography (PET) studies, net blood‐brain clearance of [(18)F]‐fluoro‐l‐DOPA (FDOPA) correlated with impaired cognitive performance in patients with Parkinson's disease or schizophrenia. We hypothesized that FDOPA influx also correlates with performance of cognitive tasks associated with prefrontal functioning in healthy volunteers. The net blood‐brain clearance of FDOPA (K [Formula: see text]) was mapped in a group of 11 healthy volunteers and calculated in striatal volumes‐of‐interest. The Wisconsin‐Card‐Sorting‐Test (WCST), Stroop‐T…

AdultMaleElementary cognitive taskMetabolic Clearance RateDopamineCaudate nucleusPrefrontal CortexStriatumNeuropsychological Testsbehavioral disciplines and activitiesSynaptic TransmissionBasal GangliaFunctional LateralityCognitionMesencephalonReference ValuesBasal gangliaNeural PathwaysReaction TimeHumansRadiology Nuclear Medicine and imagingPrefrontal cortexResearch ArticlesBrain MappingRadiological and Ultrasound TechnologyReceptors Dopamine D2PutamenCognitive flexibilityPutamenMiddle AgedDihydroxyphenylalanineNeurologyBlood-Brain BarrierPositron-Emission TomographyNeurology (clinical)AnatomyCaudate NucleusPsychologyNeuroscienceStroop effectHuman brain mapping
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The viral clearance in interferon-treated chronic hepatitis C is associated with increased cytotoxic T cell frequencies

1999

Abstract Background/Aims: Cytotoxic T lymphocytes have been demonstrated in peripheral blood and liver tissue of patients with chronic hepatitis C virus infection, but their significance for viral clearance is unknown. Therefore, we analyzed hepatitis C virus-specific cytotoxic T lymphocyte precursor frequencies in chronic hepatitis C virus carriers during interferon-α treatment. Methods: Blood mononuclear cells or CD8+ T cells from HLA-A2 positive and negative patients and controls were analyzed in chromium-release assays using a panel of 18 synthetic peptides from the HCV core, E1 and NS4 antigens bearing HLA-A2 binding motifs. Specific cytotoxic T lymphocyte precursor frequencies were st…

AdultMaleMetabolic Clearance RateHepatitis C virusBiologymedicine.disease_causeAntiviral AgentsAntigenInterferonHLA-A2 AntigenmedicineHumansCytotoxic T cellHepatologyELISPOTInterferon-alphaHepatitis CHepatitis C ChronicMiddle Agedmedicine.diseaseVirologyTreatment OutcomeCase-Control StudiesImmunologyFemaleViral loadCD8T-Lymphocytes Cytotoxicmedicine.drugJournal of Hepatology
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Population pharmacokinetic parameters of vancomycin in critically ill patients.

2006

Summary Background:  Intensive care unit patients are a highly heterogeneous population. Accurate dosing for this population requires characterization of the appropriate pharmacokinetic parameters. Objective:  To estimate population pharmacokinetic parameters of vancomycin (VAN) in adult critically ill patients and to establish the predictive performance of the resulting model. Patients and method:  Fifty critically ill patients with suspected or documented infection with VAN-sensitive micro-organisms were included. Thirty patients and 234 serum concentration–time sets obtained during clinical routine monitoring were used to estimate the pharmacokinetic parameters (group A). An open bicompa…

AdultMalePediatricsmedicine.medical_specialtyAdolescentMetabolic Clearance RatePopulationUrologyRenal functionlaw.inventionPharmacokineticslawVancomycinMedicineHumansPharmacology (medical)DosingeducationAntibacterial agentAgedRetrospective StudiesPharmacologyVolume of distributionAged 80 and overeducation.field_of_studybusiness.industryBayes TheoremMiddle AgedModels TheoreticalIntensive care unitNONMEMAnti-Bacterial AgentsIntensive Care UnitsCreatinineFemaleDrug MonitoringbusinessJournal of clinical pharmacy and therapeutics
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The enantiomers of phenprocoumon: pharmacodynamic and pharmacokinetic studies.

1976

The pharmacodynamics and pharmacokinetics of the optical enantiomers of phenprocoumon were studied in 5 normal subjects and compared to the racemic mixture. Each subject received a single oral dose of 0.6 mg/kg of racemic, S(-), and R(+) phenprocoumon. S(-) phenprocoumon was 1.6 to 2.6 times as a potent as R(+) phenprocoumon when the area under the effect/time curve was used to quantify the total anticoagulant effect per dose. Comparing the plasma concentrations that elicited the same anticoagulant effect, S(-) phenprocoumon was 1.5 to 2.5 times as potent as R(+) phenprocoumon. The anticoagulant activity of the racemic mixture was between that of the enantiomers. There was no distinct diffe…

AdultMalemedicine.drug_classMetabolic Clearance RateIn Vitro TechniquesPhenprocoumonStructure-Activity RelationshipPharmacokineticsCoumarinsmedicineHumansPharmacology (medical)Serum AlbuminPharmacologyVolume of distributionChromatographyChemistryAnticoagulantAnticoagulantsStereoisomerismHuman serum albuminKineticsPharmacodynamicsPhenprocoumonProthrombin TimeRacemic mixtureEnantiomermedicine.drugProtein BindingClinical pharmacology and therapeutics
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Transforming growth factor beta1 T29C gene polymorphism and hypertension: relationship with cardiovascular and renal damage.

2008

Distribution of T29C TGFβ1 gene polymorphism was analysed in 260 hypertensive and 134 normotensive subjects. Circulating TGFβ1 and procollagen type III levels, microalbuminuria, left ventricular geometry and function were evaluated in all the hypertensives subgrouped according to T29C TGFβ1 gene polymorphism. Circulating TGFβ1 by ELISA technique, procollagen type III by a specific radioimmunoassay, microalbuminuria by radioimmunoassay, left ventricular geometry and function by echocardiography were determined. All groups were comparable for gender, age and sex. Regarding T29C TGFβ1 gene polymorphism, prevalence of TC or CC genotypes was significantly (p < 0.05) higher in hypertensives th…

AdultMalemedicine.medical_specialtyMetabolic Clearance RateRadioimmunoassayEnzyme-Linked Immunosorbent AssayLeft ventricular hypertrophyTransforming Growth Factor beta1Procollagen type IIIElectrocardiographyInternal medicineGenotypeInternal MedicinemedicineAlbuminuriaHumansCirculating TGFβ1 Hypertension Left ventricular hypertrophy Microalbuminuria Procollagen type III TGFβ1 gene polymorphismPolymorphism GeneticRenal damagebusiness.industryRadioimmunoassayGeneral MedicineMiddle Agedmedicine.diseaseEndocrinologyCollagen Type IIICase-Control StudiesCreatinineHypertensionMicroalbuminuriaFemaleHypertrophy Left VentricularGene polymorphismCardiology and Cardiovascular MedicinebusinessProcollagenTransforming growth factorBlood pressure
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Comparison of creatinine and inulin clearances in multiple trauma

1990

Creatinine clearance (Ccr) is a good predictor of renal dysfunction. However, numerous analytical interferences have been observed with the classical measurement of creatinine by Jaffé's procedure. This prospective study was conducted to compare 4 methods for determining creatinine; and also endogenous creatinine clearance with inulin clearance (Cin) to estimate the glomerular filtration rate. The 4 different techniques for measuring creatinine were: 1) 2 techniques using Jaffé's colorimetric reaction: one with rapid and the other with slow kinetics: 2) 2 more selective methods: enzymatic procedure and high performance liquid chromatography (HPLC). Measurements were performed in 13 multiple…

AdultMalemedicine.medical_specialtyMetabolic Clearance RateUrinary systemInulinUrologyRenal functionPoison controlchemistry.chemical_compoundmedicineHumansProspective cohort studyChromatography High Pressure LiquidPharmacologyCreatinineInulin ClearanceMultiple Traumabusiness.industryInulinGeneral MedicineClinical Enzyme TestsMiddle AgedSurgerychemistryCreatinineColorimetryFemalebusinessUrine collectionBiomedicine & Pharmacotherapy
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Elevated [18F]FDOPA utilization in the periaqueductal gray and medial nucleus accumbens of patients with early Parkinson's disease

2010

Udgivelsesdato: 2010-Feb-15 PET studies with the DOPA decarboxylase substrate 6-[(18)F]fluoro-l-DOPA (FDOPA) reveal the storage of [(18)F]-fluorodopamine within synaptic vesicles, mainly of dopamine fibres. As such, FDOPA PET is a sensitive indicator of the integrity of the nigrostriatal dopamine innervation. Nonetheless, there have been several reports of focal elevations of FDOPA utilization in brain of patients with Parkinson's disease (PD), all based on reference tissue methods. To investigate this phenomenon further, we used voxel-wise steady-state kinetic analysis to search for regions of elevated FDOPA utilization (K; ml g(-1) min(-1)) and steady-state trapping (V(d); ml g(-1)) in a …

AdultMalemedicine.medical_specialtyParkinson's diseaseMetabolic Clearance RateCognitive NeuroscienceStriatumNucleus accumbensPeriaqueductal grayNucleus AccumbensDopamineInternal medicinemedicineHumansPeriaqueductal GrayAgedAromatic L-amino acid decarboxylasebusiness.industryParkinsonismPutamenParkinson DiseaseMiddle Agedmedicine.diseaseDihydroxyphenylalanineEndocrinologyNeurologyPositron-Emission TomographyFemaleRadiopharmaceuticalsbusinessNeurosciencemedicine.drugNeuroImage
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Estimation of pharmacokinetic parameters of lithium from saliva and urine

1974

The salivary and urinary excretion of lithium was studied in three healthy male sub;ects after oral administration of two or three different doses. In all individuals the concentration of lithium in salivary fluid was found to be 2.2 to 3.3 times as high as the concentration in plasma. In each sub;ect the saliva:plasma concentration ratio remained constant over more than a 100 fold concentration range for at least 3 months. This ratio was not markedly affected by about tenfold changes in saliva flow rate. Thus, pharmacokinetic parameters obtained from salivary excretion data are in agreement with those obtained from plasma concentration and urinary excretion rate data, and renal clearance o…

AdultMalemedicine.medical_specialtySalivaTime FactorsSalivary ExcretionMetabolic Clearance RateAdministration OralUrineLithiumKidneyUrinary excretionPharmacokineticsOral administrationInternal medicinemedicineHumansPharmacology (medical)SalivaPharmacologyChemistrySpectrophotometry AtomicDiurnal rhythmsCircadian RhythmKineticsEndocrinologyCreatinineRegression AnalysisSalivationHalf-LifeClearanceClinical Pharmacology & Therapeutics
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